How to Get Concentration from Absorbance: A Simple Guide for Absolute Beginners

How To Get Concentration From Absorbance

How to Get Concentration from Absorbance: A Simple Guide for Absolute Beginners

Concentration is a measure of the amount of a substance in a given volume of solution. It is often expressed in units of moles per liter (M). Absorbance is a measure of the amount of light that is absorbed by a solution. It is often expressed in units of absorbance units (AU). The relationship between concentration and absorbance is described by the Beer-Lambert law, which states that the absorbance of a solution is directly proportional to the concentration of the absorbing substance and the path length of the light beam through the solution.

The Beer-Lambert law can be used to determine the concentration of a substance in a solution by measuring the absorbance of the solution at a specific wavelength. This technique is often used in analytical chemistry to determine the concentration of unknown substances in solution.

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